diff --git a/packages/ui-patterns/src/FilterBar/CommandListItem.tsx b/packages/ui-patterns/src/FilterBar/CommandListItem.tsx
index 774d92e5ae4..e0fd1199a5b 100644
--- a/packages/ui-patterns/src/FilterBar/CommandListItem.tsx
+++ b/packages/ui-patterns/src/FilterBar/CommandListItem.tsx
@@ -1,3 +1,4 @@
+import { Check } from 'lucide-react'
 import { cn } from 'ui'
 
 import { OperatorSymbolBadge } from './OperatorSymbolBadge'
@@ -8,6 +9,7 @@ export type CommandListItemProps = {
   item: MenuItem
   isHighlighted: boolean
   includeIcon: boolean
+  showSelection?: boolean
   onSelect: (item: MenuItem) => void
   setRef: (el: HTMLDivElement | null) => void
 }
@@ -16,6 +18,7 @@ export function CommandListItem({
   item,
   isHighlighted,
   includeIcon,
+  showSelection = false,
   onSelect,
   setRef,
 }: CommandListItemProps) {
@@ -23,15 +26,28 @@ export function CommandListItem({
     <div
       ref={setRef}
       role="option"
-      onClick={() => onSelect(item)}
+      aria-disabled={item.disabled}
+      onClick={() => {
+        if (!item.disabled) onSelect(item)
+      }}
       className={cn(
-        'relative flex items-center justify-between gap-2 px-2 h-[28px] text-xs cursor-pointer select-none outline-hidden text-foreground',
+        'relative flex items-center justify-between gap-2 px-2 h-[28px] text-xs select-none outline-hidden',
+        item.disabled ? 'text-muted' : 'text-foreground cursor-pointer',
         isHighlighted && 'bg-overlay-hover',
-        !isHighlighted && 'hover:bg-surface-200'
+        !isHighlighted && !item.disabled && 'hover:bg-surface-200'
       )}
       data-testid={`filter-menu-item-${item.value}`}
     >
       <span className="flex items-center gap-2 min-w-0">
+        {showSelection && (
+          <>
+            {item.disabled ? (
+              <Check className="w-3.5 h-3.5 shrink-0 text-foreground-muted" />
+            ) : (
+              <div className="w-3.5" />
+            )}
+          </>
+        )}
         {includeIcon && item.icon}
         <span className="truncate">{getActionItemLabel(item)}</span>
       </span>
diff --git a/packages/ui-patterns/src/FilterBar/DefaultCommandList.tsx b/packages/ui-patterns/src/FilterBar/DefaultCommandList.tsx
index 761019dfc25..a84e996a17a 100644
--- a/packages/ui-patterns/src/FilterBar/DefaultCommandList.tsx
+++ b/packages/ui-patterns/src/FilterBar/DefaultCommandList.tsx
@@ -12,6 +12,7 @@ export type DefaultCommandListProps = {
   highlightedIndex: number
   onSelect: (item: MenuItem) => void
   includeIcon?: boolean
+  showSelection?: boolean
   grouped?: boolean
 }
 
@@ -20,6 +21,7 @@ export function DefaultCommandList({
   highlightedIndex,
   onSelect,
   includeIcon = true,
+  showSelection = false,
   grouped = false,
 }: DefaultCommandListProps) {
   const listRef = useRef<HTMLDivElement>(null)
@@ -73,6 +75,7 @@ export function DefaultCommandList({
           )}
           {groupData.items.map(({ item, index }) => (
             <CommandListItem
+              showSelection={showSelection}
               key={`${item.value}-${item.label}`}
               item={item}
               isHighlighted={index === highlightedIndex}
diff --git a/packages/ui-patterns/src/FilterBar/FilterCondition.tsx b/packages/ui-patterns/src/FilterBar/FilterCondition.tsx
index 928bf4be725..f84e68fa284 100644
--- a/packages/ui-patterns/src/FilterBar/FilterCondition.tsx
+++ b/packages/ui-patterns/src/FilterBar/FilterCondition.tsx
@@ -215,7 +215,7 @@ export function FilterCondition({
     valueItems.length,
     (index) => {
       const item = valueItems[index]
-      if (!item) return
+      if (!item || item.disabled) return
       if (item.isCustom) {
         setShowValueCustom(true)
       } else {
@@ -421,11 +421,13 @@ export function FilterCondition({
             })
           ) : (
             <DefaultCommandList
+              showSelection
               items={valueItems}
               highlightedIndex={valHighlightedIndex}
-              onSelect={(item) =>
+              onSelect={(item) => {
+                if (item.disabled) return
                 item.isCustom ? setShowValueCustom(true) : handleSelectMenuItem(item)
-              }
+              }}
               includeIcon
             />
           )}
diff --git a/packages/ui-patterns/src/FilterBar/menuItems.test.ts b/packages/ui-patterns/src/FilterBar/menuItems.test.ts
index 52c1a95b984..ba5358c7052 100644
--- a/packages/ui-patterns/src/FilterBar/menuItems.test.ts
+++ b/packages/ui-patterns/src/FilterBar/menuItems.test.ts
@@ -161,6 +161,31 @@ describe('buildValueItems', () => {
     ])
   })
 
+  it('disables options already used by another condition on the same property', () => {
+    const filters: FilterGroup = {
+      logicalOperator: 'AND',
+      conditions: [
+        { propertyName: 'name', operator: '=', value: 'alice' },
+        { propertyName: 'name', operator: '=', value: '' },
+      ],
+    }
+
+    const items = buildValueItems(
+      { type: 'value', path: [1] },
+      filters,
+      filterProperties,
+      {},
+      {},
+      '',
+      false
+    )
+
+    expect(items).toEqual([
+      { value: 'alice', label: 'Alice', disabled: true },
+      { value: 'bob', label: 'Bob' },
+    ])
+  })
+
   it.each(['~~*', '!~~*'])(
     'suppresses value suggestions for pattern-group operator `%s`',
     (operator) => {
diff --git a/packages/ui-patterns/src/FilterBar/menuItems.ts b/packages/ui-patterns/src/FilterBar/menuItems.ts
index 8ed83c386d2..3031b95e00a 100644
--- a/packages/ui-patterns/src/FilterBar/menuItems.ts
+++ b/packages/ui-patterns/src/FilterBar/menuItems.ts
@@ -1,9 +1,11 @@
 import { ActiveInputState, FilterBarAction, FilterGroup, FilterProperty, MenuItem } from './types'
 import {
+  collectConditions,
   findConditionByPath,
   isCustomOptionObject,
   isFilterOperatorObject,
   isFilterOptionObject,
+  pathsEqual,
 } from './utils'
 
 export function buildOperatorItems(
@@ -142,8 +144,16 @@ export function buildValueItems(
 
   if (!property) return items
 
+  // Values already used by other conditions on this same property shouldn't be re-selectable.
+  const usedValues = new Set(
+    collectConditions(activeFilters)
+      .filter(({ path }) => !pathsEqual(path, activeInput.path))
+      .filter(({ condition }) => condition.propertyName === activeCondition?.propertyName)
+      .map(({ condition }) => String(condition.value))
+  )
+
   if (activeCondition?.operator === 'is') {
-    return getIsOperatorValueItems(property, inputValue, hasTypedSinceFocus)
+    return getIsOperatorValueItems(property, inputValue, hasTypedSinceFocus, usedValues)
   }
 
   // Pattern-matching operators (e.g. iLike) search for a substring, not an exact
@@ -174,10 +184,11 @@ export function buildValueItems(
         inputValue,
         hasTypedSinceFocus,
         showCount: activeCondition?.operator === '=',
+        usedValues,
       })
     )
   } else if (propertyOptionsCache[property.name]) {
-    items.push(...getCachedOptionItems(propertyOptionsCache[property.name].options))
+    items.push(...getCachedOptionItems(propertyOptionsCache[property.name].options, usedValues))
   }
 
   return items
@@ -188,11 +199,13 @@ function getArrayOptionItems({
   inputValue,
   hasTypedSinceFocus,
   showCount,
+  usedValues,
 }: {
   options: any[]
   inputValue: string
   hasTypedSinceFocus: boolean
   showCount?: boolean
+  usedValues?: Set<string>
 }): MenuItem[] {
   const items: MenuItem[] = []
   const normalizedInput = inputValue.toLowerCase()
@@ -203,7 +216,7 @@ function getArrayOptionItems({
   for (const option of options) {
     if (typeof option === 'string') {
       if (!shouldFilter || option.toLowerCase().includes(normalizedInput)) {
-        items.push({ value: option, label: option })
+        items.push({ value: option, label: option, disabled: usedValues?.has(option) || undefined })
       }
     } else if (isFilterOptionObject(option)) {
       if (!shouldFilter || option.label.toLowerCase().includes(normalizedInput)) {
@@ -211,6 +224,7 @@ function getArrayOptionItems({
           value: option.value,
           label: option.label,
           count: showCount ? option.count : undefined,
+          disabled: usedValues?.has(option.value) || undefined,
         })
       }
     } else if (isCustomOptionObject(option)) {
@@ -227,19 +241,24 @@ function getArrayOptionItems({
   return items
 }
 
-function getCachedOptionItems(options: any[]): MenuItem[] {
+function getCachedOptionItems(options: any[], usedValues?: Set<string>): MenuItem[] {
   return options.map((option) => {
     if (typeof option === 'string') {
-      return { value: option, label: option }
+      return { value: option, label: option, disabled: usedValues?.has(option) || undefined }
+    }
+    return {
+      value: option.value,
+      label: option.label,
+      disabled: usedValues?.has(option.value) || undefined,
     }
-    return { value: option.value, label: option.label }
   })
 }
 
 function getIsOperatorValueItems(
   property: FilterProperty,
   inputValue: string,
-  hasTypedSinceFocus: boolean
+  hasTypedSinceFocus: boolean,
+  usedValues?: Set<string>
 ): MenuItem[] {
   const options: { value: string; label: string }[] = [
     { value: 'null', label: 'NULL' },
@@ -251,11 +270,14 @@ function getIsOperatorValueItems(
   }
 
   const shouldFilter = hasTypedSinceFocus && inputValue.length > 0
-  if (!shouldFilter) return options
+  const filtered = shouldFilter
+    ? options.filter((opt) => {
+        const normalizedInput = inputValue.toLowerCase()
+        return (
+          opt.label.toLowerCase().includes(normalizedInput) || opt.value.includes(normalizedInput)
+        )
+      })
+    : options
 
-  const normalizedInput = inputValue.toLowerCase()
-  return options.filter(
-    (opt) =>
-      opt.label.toLowerCase().includes(normalizedInput) || opt.value.includes(normalizedInput)
-  )
+  return filtered.map((opt) => ({ ...opt, disabled: usedValues?.has(opt.value) || undefined }))
 }
diff --git a/packages/ui-patterns/src/FilterBar/types.ts b/packages/ui-patterns/src/FilterBar/types.ts
index 5a1a29b3216..af18893e0eb 100644
--- a/packages/ui-patterns/src/FilterBar/types.ts
+++ b/packages/ui-patterns/src/FilterBar/types.ts
@@ -104,6 +104,7 @@ export type MenuItem = {
   isFreeformSearch?: boolean
   freeformPropertyName?: string
   freeformValue?: string
+  disabled?: boolean
 }
 
 export type GroupedMenuItem = {
diff --git a/packages/ui-patterns/src/FilterBar/utils.ts b/packages/ui-patterns/src/FilterBar/utils.ts
index c89a39e846b..be7e9769396 100644
--- a/packages/ui-patterns/src/FilterBar/utils.ts
+++ b/packages/ui-patterns/src/FilterBar/utils.ts
@@ -56,6 +56,18 @@ export function findConditionByPath(group: FilterGroup, path: number[]): FilterC
   return null
 }
 
+export function collectConditions(
+  group: FilterGroup,
+  path: number[] = []
+): { condition: FilterCondition; path: number[] }[] {
+  return group.conditions.flatMap((condition, index) => {
+    const currentPath = [...path, index]
+    return isGroup(condition)
+      ? collectConditions(condition, currentPath)
+      : [{ condition, path: currentPath }]
+  })
+}
+
 export function isCustomOptionObject(option: any): option is CustomOptionObject {
   return typeof option === 'object' && option !== null && 'component' in option
 }